Journal: FEBS Open Bio

Article Title: Selective estrogen receptor modulators decrease invasiveness in pituitary adenoma cell lines AtT‐20 and TtT/GF by affecting expression of MMP‐14 and ADAM12

doi: 10.1002/2211-5463.12999

Figure Lengend Snippet: Selective estrogen receptor modulators reduce invasiveness in PA cell lines AtT‐20 (B) and TtT/GF (C). (A) Representative images of AtT20 (upper panel) and TtT/GF (lower panel) cells stained with DAPI. Note that cell lines were treated with 25% inhibitory concentrations (IC 25 ) of SERMs bazedoxifene, clomiphene, and raloxifene as indicated in Table . Scale bar in (A), for all images, 50 μm. Batimastat, a broad‐range MMP inhibitor, served as positive control. With bazedoxifene in TtT/GF cells, the outlier (o) was excluded from the statistical analysis. Values are given as median, first quartile, and third quartile. Values are obtained from three independent experiments performed in triplicates. t served as statistical test. *Statistically significant difference as compared to control.

Article Snippet: Selective estrogen receptor modulators (bazedoxifene, clomiphene, and raloxifene) were purchased from Biomol (Hamburg, Germany).

Techniques: Staining, Positive Control, Control